Pcr based detection of zoonotic salmonella from foods jan 04 2020 posted by andrew neiderman public library text id 4535a213 online pdf ebook epub library for foodborne disease surveillance and outbreak investigations traditional immunological techniques are labor intensive and time consuming whereas polymerase chain r. Detection of salmonella spp presence in food real time pcr for detection of salmonella in food should be carefully followed during the preparation of fresh poultry meat based food items. Polymerase chain reaction pcr and more recently real time pcr assays have been developed for the detection of salmonellae or specific serotypes in a variety of foods 9 11 several pcr assays have been developed by targeting various salmonella genes such as 16s rrna 12 agfa 13 viab 14 and virulence associated plasmids 15 . Kits for salmonella detection in foods chromogenic media pcr immunoassay 2002 for the detection salmonella spp in food and animal feed the method was amended in 2007 to include testing of animal faeces and environmental samples from primary production and molecular techniques such as dna hybridisation and pcr based assays many . Detection of salmonella in food matrices from conventional methods to recent aptamer sensing non typhoid human salmonellosis is considered to be a zoonotic disease polymerase chain reaction pcr based methods have also been applied for the detection and
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